Sample ID: VE24-1351_COI
[analysis description + link to github repo]
| Facility | Hogwarts |
| Analyst | John Doe |
| Analysis started | 2025-05-03 01:28:01 |
| Analysis completed | 2025-05-03 01:28:01 |
| Wall time | 0:0:0 hours |
Inconclusive
Outcome: The analyst should attempt subjective species identification at the genus level.
Reasoning: [Flag 1B] 2-3 candidate species matched with high stringency (identity ≥ 98.5%).
| Preliminary morphology ID confirmed? | NA |
|
Inconclusive taxonomic identity (Flag 1B) Sciaridae. |
| Taxa of interest detected? | True |
|
Flag 2A: Taxon of interest detected in candidate species Flag 5.1A: The given locus for this taxon is well represented in reference database (>5 entries) Flag 5.2B: 10-90% of related taxa have reference sequence(s) at the given locus |
|
| Locus | COI |
| Preliminary ID | Sciaridae |
| Taxa of interest |
Lycoriella ingenua |
| Country | Netherlands |
| Host | Lilium bulbs |
| Sample ID | VE24-1351_COI |
| Query DNA sequence |
Flag 1B:
The analyst should attempt subjective species identification at the genus level
2-3 candidate species matched with high stringency (identity ≥ 98.5%)
Candidate hits must meet ONE of these criteria:
| Minimum alignment length |
400bp
|
| Minimum query coverage |
85.0%
|
Candidate hits have then been classified as follows:
| Classification | Alignment identity | Number of hits | Number of species |
|---|---|---|---|
| STRONG MATCH | ≥ 98.5% | 31 | 3 |
| MODERATE MATCH | ≥ 93.5% | NA | NA |
| NO MATCH | < 93.5% |
| Species | Hits | Identity | E-value | Database coverage |
|---|---|---|---|---|
| Bradysia impatiens | 2 | 100.0% | 0.0 | |
| Lycoriella ingenua | 27 | 100.0% | 0.0 | |
| Lycoriella mali | 2 | 100.0% | 0.0 |
This sections shows the taxa of interest (TOI) specified by the sample submitter. Each of these taxa has been cross-referenced against the candidate species to determine if they might match the taxonomic identity of the sample. A blank row indicates a TOI that did not match any candidate species, meaning that it is unlikely that the sample matches that TOI.
| Taxon of interest | Match rank | Match taxon | Match species | Match accession | Match identity | Database coverage |
|---|---|---|---|---|---|---|
| Lycoriella ingenua | species | Lycoriella ingenua | Lycoriella ingenua | PQ008449 | 1.0 |
This analysis evaluates how many independent sources have contributed to reference sequences for each candidate species. This provides a measure of confidence in the taxonomic annotation of references sequences. A sequence that has been annotated by multiple independent sources is more likely to have a correct taxonomic annotation.
Independent sources
Flag 4B:
Reference sequence sources lack diversity and may therefore be unreliable
Reasoning: Matching sequence records for this species have only 1-5 independent sources
(found 1 sources)
1 Independent Source
The matching reference sequences for this species have been annotated by 1 independent source(s). A source is considered independent if the author list or publication title is distinct.
Source 1
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| MK861019 | False |
Katumanyane,A. Kanzi,A.M. Malan,A.P. |
Fungus gnats in South Africa | Unpublished |
| MK861019 | False |
Katumanyane,A. Kanzi,A.M. Malan,A.P. |
Direct Submission | Submitted (30-APR-2019) Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Lunnon and Herold Street, Hatfiled, Pretoria 0002, South Africa |
| MK861018 | False |
Katumanyane,A. Kanzi,A.M. Malan,A.P. |
Fungus gnats in South Africa | Unpublished |
| MK861018 | False |
Katumanyane,A. Kanzi,A.M. Malan,A.P. |
Direct Submission | Submitted (30-APR-2019) Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Lunnon and Herold Street, Hatfiled, Pretoria 0002, South Africa |
| Show 2 more publications... | ||||
Independent sources
Flag 4A:
Reference sequences are from diverse sources and therefore likely to be reliable
Reasoning: Matching sequence records for this species are from >5 independent sources
(found 11 sources)
11 Independent Sources
The matching reference sequences for this species have been annotated by 11 independent source(s). A source is considered independent if the author list or publication title is distinct.
Source 1
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| PQ008449 | False |
Ma,L. Lin,J. |
Direct Submission | Submitted (11-JUL-2024) Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling, Nanjing 210014, China |
| PQ069699 | False |
Ma,L. Lin,J. |
Direct Submission | Submitted (24-JUL-2024) Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling, Nanjing 210014, China |
| PQ066142 | False |
Ma,L. Lin,J. |
Direct Submission | Submitted (24-JUL-2024) Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling, Nanjing 210014, China |
| PQ069630 | False |
Ma,L. Lin,J. |
Direct Submission | Submitted (24-JUL-2024) Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling, Nanjing 210014, China |
| PQ069628 | False |
Ma,L. Lin,J. |
Direct Submission | Submitted (24-JUL-2024) Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling, Nanjing 210014, China |
| Show 3 more publications... | ||||
Source 2
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| JN378570 | False |
Shin,S. Jung,S. Lee,H. Lee,S. |
Molecular identification of dipteran pests (Diptera: Sciaroidea) from shiitake mushroom | Mol Ecol Resour 13 (2), 200-209 (2013) |
| JN378570 | False |
Shin,S. Lee,H. Jung,S. Lee,S. |
Direct Submission | Submitted (20-JUL-2011) Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea |
Source 3
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| KX360302 | False |
Ye,L. Leng,R. Huang,J. |
Review of three greenhouse pest species of Black Fungus Gnats (Diptera:Sciaridae) from China | Unpublished |
| KX360302 | False |
Ye,L. Leng,R. Huang,J. |
Direct Submission | Submitted (06-JUN-2016) Department of Forestry Protection, Zhejiang A & F University, Lin'an, Hangzhou, Zhejiang 311300, China |
| KX360295 | False |
Ye,L. Leng,R. Huang,J. |
Review of three greenhouse pest species of Black Fungus Gnats (Diptera:Sciaridae) from China | Unpublished |
| KX360295 | False |
Ye,L. Leng,R. Huang,J. |
Direct Submission | Submitted (06-JUN-2016) Department of Forestry Protection, Zhejiang A & F University, Lin'an, Hangzhou, Zhejiang 311300, China |
| KX360299 | False |
Ye,L. Leng,R. Huang,J. |
Review of three greenhouse pest species of Black Fungus Gnats (Diptera:Sciaridae) from China | Unpublished |
| KX360299 | False |
Ye,L. Leng,R. Huang,J. |
Direct Submission | Submitted (06-JUN-2016) Department of Forestry Protection, Zhejiang A & F University, Lin'an, Hangzhou, Zhejiang 311300, China |
| KX360301 | False |
Ye,L. Leng,R. Huang,J. |
Review of three greenhouse pest species of Black Fungus Gnats (Diptera:Sciaridae) from China | Unpublished |
| KX360301 | False |
Ye,L. Leng,R. Huang,J. |
Direct Submission | Submitted (06-JUN-2016) Department of Forestry Protection, Zhejiang A & F University, Lin'an, Hangzhou, Zhejiang 311300, China |
| KX360300 | False |
Ye,L. Leng,R. Huang,J. |
Review of three greenhouse pest species of Black Fungus Gnats (Diptera:Sciaridae) from China | Unpublished |
| KX360300 | False |
Ye,L. Leng,R. Huang,J. |
Direct Submission | Submitted (06-JUN-2016) Department of Forestry Protection, Zhejiang A & F University, Lin'an, Hangzhou, Zhejiang 311300, China |
| KX360292 | False |
Ye,L. Leng,R. Huang,J. |
Review of three greenhouse pest species of Black Fungus Gnats (Diptera:Sciaridae) from China | Unpublished |
| KX360292 | False |
Ye,L. Leng,R. Huang,J. |
Direct Submission | Submitted (06-JUN-2016) Department of Forestry Protection, Zhejiang A & F University, Lin'an, Hangzhou, Zhejiang 311300, China |
| KX360315 | False |
Ye,L. Leng,R. Huang,J. |
Review of three greenhouse pest species of Black Fungus Gnats (Diptera:Sciaridae) from China | Unpublished |
| KX360315 | False |
Ye,L. Leng,R. Huang,J. |
Direct Submission | Submitted (06-JUN-2016) Department of Forestry Protection, Zhejiang A & F University, Lin'an, Hangzhou, Zhejiang 311300, China |
| KX360291 | False |
Ye,L. Leng,R. Huang,J. |
Review of three greenhouse pest species of Black Fungus Gnats (Diptera:Sciaridae) from China | Unpublished |
| KX360291 | False |
Ye,L. Leng,R. Huang,J. |
Direct Submission | Submitted (06-JUN-2016) Department of Forestry Protection, Zhejiang A & F University, Lin'an, Hangzhou, Zhejiang 311300, China |
| Show 14 more publications... | ||||
Source 4
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| FN868635 | False |
Andrew King,R. Moreno-Ripoll,R. Agusti,N. Shayler,S.P. Bell,J.R. Bohan,D.A. Symondson,W.O. |
Multiplex reactions for the molecular detection of predation on pest and nonpest invertebrates in agroecosystems | Mol Ecol Resour 11 (2), 370-373 (2011) |
| FN868635 | False | King,A. | Direct Submission | Submitted (19-MAY-2010) King A., School of Biosciences, University of Exeter, Stocker Road, EX4 4QD, UNITED KINGDOM |
Source 5
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| OR352396 | False |
Yang,F. Liu,H. |
A rapid multiplex PCR assay for the identification of black fungus gnats (Diptera: Sciaridae) and its application to larvae in mushrooms | Unpublished |
| OR352396 | False |
Yang,F. Liu,H. |
Direct Submission | Submitted (26-JUL-2023) Youth Research Group, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, No. 9, Shuguang Garden Middle Road, Beijing 100097, China |
| OR690424 | False |
Yang,F. Liu,H. |
Direct Submission | Submitted (18-OCT-2023) Youth Research Group, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, No. 9, Shuguang Garden Middle Road, Beijing 100097, China |
| Show 1 more publication... | ||||
Source 6
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| MT252095 | False |
Karademir,G.K. Yildirim,A. Inci,A. Tuncbilek,A.S. |
Molecular Characterization of Lycoriella ingenua Lineges in Central Anatolia Region of Turkey | Unpublished |
| MT252095 | False |
Karademir,G.K. Yildirim,A. Inci,A. Tuncbilek,A.S. |
Direct Submission | Submitted (27-MAR-2020) Parasitology, Erciyes University Faculty of Veterinary Medicine, Talas, Kayseri 38039, Turkey |
Source 7
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| LC278318 | False |
Arimoto,M. Uesugi,R. Hinomoto,N. Sueyoshi,M. Yoshimatsu,S. |
Molecular marker to identify the fungus gnat, Bradysia sp. (Diptera: Sciaridae), a new pest of Welsh onion and carrot in Japan | Appl. Entomol. Zool. (Jpn.) 53, 419-424 (2018) |
| LC278318 | False |
Arimoto,M. Uesugi,R. Hinomoto,H. |
Direct Submission | Submitted (15-JUN-2017) Contact:Makoto Arimoto Central Region Agricultural Research Center, NARO, Division of Applied Entomology and Zoology; 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8666, Japan URL :http://www.naro.affrc.go.jp/english/narc/index.html |
Source 8
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| OR251912 | False |
Ma,L. Qu,S. Lin,J. |
Investigation of main fungus pests on Auricularia and screening of insecticides | Unpublished |
| OR251912 | False |
Ma,L. Qu,S. Lin,J. |
Direct Submission | Submitted (11-JUL-2023) Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling, Nanjing 210014, China |
| OR253027 | False |
Ma,L. Qu,S. Lin,J. |
Investigation of main fungus pests on Auricularia and screening of insecticides | Unpublished |
| OR253027 | False |
Ma,L. Qu,S. Lin,J. |
Direct Submission | Submitted (11-JUL-2023) Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling, Nanjing 210014, China |
| Show 2 more publications... | ||||
Source 9
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| MZ021517 | False |
Hassan,A.A. Al-Qaissi,A.R. |
The first record of the mushroom flies Megaselia halterata and Lycoriella ingenua in Iraq and the production of environmentally friendly attractants for these insect traps | Unpublished |
| MZ021517 | False |
Hassan,A.A. Al-Qaissi,A.R. |
Direct Submission | Submitted (24-APR-2021) Plant protection, Tikrit university, 73, Tikrit, Salah Aldin 00964, Iraq |
Source 10
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| MG291873 | False | Dewaard,J.R. | BIOUG Archive GGBN Data Release | Unpublished |
| MG291873 | False | Dewaard,J.R. | Direct Submission | Submitted (30-OCT-2017) Collections, Centre for Biodiversity Genomics, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada |
| MG292424 | False | Dewaard,J.R. | BIOUG Archive GGBN Data Release | Unpublished |
| MG292424 | False | Dewaard,J.R. | Direct Submission | Submitted (30-OCT-2017) Collections, Centre for Biodiversity Genomics, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada |
| MG159463 | False | Dewaard,J.R. | BIOUG Archive GGBN Data Release | Unpublished |
| MG159463 | False | Dewaard,J.R. | Direct Submission | Submitted (11-OCT-2017) Collections, Centre for Biodiversity Genomics, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada |
| Show 4 more publications... | ||||
Source 11
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| KR746145 | False |
Hebert,P.D. Ratnasingham,S. Zakharov,E.V. Telfer,A.C. Levesque-Beaudin,V. Milton,M.A. Pedersen,S. Jannetta,P. deWaard,J.R. |
Counting animal species with DNA barcodes: Canadian insects | Philos. Trans. R. Soc. Lond., B, Biol. Sci. 371 (1702) (2016) In press |
| KR746145 | False |
Hebert,P.D.N. Ratnasingham,S. Zakharov,E.V. Dewaard,J.R. |
Direct Submission | Submitted (15-MAY-2015) Biodiversity Institute of Ontario, University of Guelph, 50 Stone Road EAST, Guelph, Ontario N1G2W1, Canada |
| KR761633 | False |
Hebert,P.D. Ratnasingham,S. Zakharov,E.V. Telfer,A.C. Levesque-Beaudin,V. Milton,M.A. Pedersen,S. Jannetta,P. deWaard,J.R. |
Counting animal species with DNA barcodes: Canadian insects | Philos. Trans. R. Soc. Lond., B, Biol. Sci. 371 (1702) (2016) In press |
| KR761633 | False |
Hebert,P.D.N. Ratnasingham,S. Zakharov,E.V. Dewaard,J.R. |
Direct Submission | Submitted (15-MAY-2015) Biodiversity Institute of Ontario, University of Guelph, 50 Stone Road EAST, Guelph, Ontario N1G2W1, Canada |
| Show 2 more publications... | ||||
Independent sources
Flag 4B:
Reference sequence sources lack diversity and may therefore be unreliable
Reasoning: Matching sequence records for this species have only 1-5 independent sources
(found 1 sources)
1 Independent Source
The matching reference sequences for this species have been annotated by 1 independent source(s). A source is considered independent if the author list or publication title is distinct.
Source 1
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| AF319784 | False |
Bae,J.S. Kim,I. Kim,S.R. Jin,B.R. Sohn,H.D. |
Mitochondrial DNA sequence variation of the mushroom pest flies, Lycoriella mali (Diptera: Sciaridae) and Coboldia fuscipes (Diptera: Scatopsidae) in Korea | Appl. Entomol. Zool. (Jpn.) 36, 451-457 (2001) |
| AF319784 | False | Bae,J.-S. | Direct Submission | Submitted (08-NOV-2000) Dong-A University, 840 Hadan2-dong, Saha-gu, Busan, Korea |
| AF319811 | False |
Bae,J.S. Kim,I. Kim,S.R. Jin,B.R. Sohn,H.D. |
Mitochondrial DNA sequence variation of the mushroom pest flies, Lycoriella mali (Diptera: Sciaridae) and Coboldia fuscipes (Diptera: Scatopsidae) in Korea | Appl. Entomol. Zool. (Jpn.) 36, 451-457 (2001) |
| AF319811 | False | Bae,J.-S. | Direct Submission | Submitted (08-NOV-2000) Dong-A University, 840 Hadan2-dong, Saha-gu, Busan, Korea |
| Show 2 more publications... | ||||
This section provides a phylogeny of the candidate reference sequences. The analyst can use this to make a subjective observation on how well the reference sequences are able to distinguish between species. If the phylogeny shows distinct clades for each species, we can be confident that the molecular data are capable of distinguishing between those species. However, if the phylogeny shows overlap between species, this reduces the capacity of the molecular data to confidently distinguish between those species. In some cases, we may see the query sequence falling outside of the adjacent species' clades, which indicates that our query species is not represented in the reference database, which could indicate a rare or novel species.
The following resources can be used to ensure that the given taxonomy is legitimate and current.
| Taxa | Database |
|---|---|
| General | GBIF |
| General | ITIS |
| Mealybugs & scale | ScaleNet database |
| Thrips | Thripswiki |
| Spider Mites | Spider Mites Database |
| Psocodea (Barklice, Booklice, and Parasitic Lice) | Psocodea Species File Online |
| Orthoptera | Orthoptera Species File Online |
| Drosophilidae | TaxoDros |
| Diptera |
Catalog of the Diptera of the Australasian and Oceanian Regions
Systema Dipterorum |
| Aphids | Aphid Species File |
| Ants |
AntWeb
AntCat |
| Lepidoptera (butterflies and moths) | The Global Lepidoptera Names Index |
| Gracillariidae (primitive moths) | Global Taxonomic Database of Gracillariidae |
| Pyralidae (pyralid moths) | Global Information System on Pyraloidea |
| Tortricidae (tortrix moths) | Tortricidae Resources on the Net |